Basic Medical Science Research Institute of China’s Academy of Medical Science
Shan Huimin Zhou Yuanzheng Huang Manying
Han Xiaosi Xu Rongkun
Zhineng Qigong Beijing Branch Guo Jinying
In this experiment, normal male Sprague-Dawley(S.D.) rats are taken as experimental subjects and the radioactive immunity method is used in measuring the content of prolactin in their plasma (PRL) in order to observe qigong’s effect of external qi on the secretion of prolactin in the resting rats or in the rats in a restricted stress reaction state. The result of the experiment shows that qigong external qi can evidently increase the content of PRL in the plasma of resting rats; while the level of PRL after the restricted stress reaction, instead of going up, goes slightly down. This shows that qigong external qi can enhance the rats’ secretion of PRL when they are in a still state. It can also inhibit their release of PRL when they are in a restricted stress reaction state.
Qigong external qi, resting, restricted stress reaction, radioactive immunity, prolactin in plasma
It has been reported that qigong external qi can inhibit the mitosis of the tumor cells cultured outside the human body (1) and can reduce the blood sugar concentration in experimental diabetic rats (2). However, the influence of external qi on the secretion of hormone in normal resting rats and in the rats in a stress reaction state has not been reported yet. It is already known that the secretion of prolactin in human beings’ and in animals’ anterior lobe of pituitary gland is liable to be affected by the condition in the outside world. Therefore, in this experiment, normal rats are used as experimental subjects to observe whether qigong external qi can affect prolactin (PRL) in resting rats and in the rats in restricted stress reaction.
Materials and Method
Experiment on the animal:
17 male Sprague-Dawley (S.D.) rats (provided by Animal Research Laboratory of Military Academy of Medical Science) are used in this experiment. They are 240g to 260g in weight and are divided into two big groups. The first big group receives qi from the qigong master when the rats are in a calm state. Blood is taken from them both before and after the qi transmission. The second big group receives qi from the qigong master when they are in a restricted stress reaction state. Blood is taken from them before the stress reaction and also after the stress reaction that is accompanied with the qigong master’s external qi transmission.
On the day before the experiment, using the method previously reported(3), prepare the silicon rubber duct and fix it to each of the rats from the vein on their neck to their right atrium so as to take blood from the clear-headed rats that are in free activity.
Between 10am to 11am on the day of the experiment, conduct and complete the experiment on the rats according to the following procedure: Group One (five rats): Take blood sample respectively from each rat and then the qigong master transmits qi to them; take blood sample respectively again from each of them 5m to 10m after the qi transmission. Group Two: This group is further divided into two small groups (6 rats in each group). Take blood sample from them both before and after the stress reaction. Then immediately do restricted stress reaction experiment on the two small groups of rats (Put them into specially made plexiglass cover until the end of the experiment). When this is over, take blood sample from each rat in the first small group (the control group in which no qigong is used). The second small group, having experienced the restricted stress reaction, receives qi from the qigong master. When the qi transmission is over, blood sample is taken from each of them. The amount of blood taken each time from each of these rats is about 1.2ml. Heparin (20IU/ml) normal saline of the same amount is supplemented to these animals after the blood sample is taken from them. The blood sample (heparin anticoagulation) is centrifugalized (3000 rpm, 20min, 40C) and blood plasma is obtained. The blood plasma is kept in the refrigerator at -200 C. It is so kept ready for measuring its hormone content.
Measuring the hormone content in the blood plasma:
With the method we have reported(4), measure the PRL content with the radioactive immunity method. The radioactive immunity measurement box that measures PRL is provided by NLADDK of America.
Data processing and statistical analysis:
The numerical value is indicated with “the average value±standard deviation (x±se)”; T-test is adopted in checking the significance of the difference in the numerical value.
1． The influence of qigong external qi on the secretion of PRL of resting rats
Qigong master transmits qi to the five rats in the group that are in a calm state with the mental instruction of increasing their PRL. As a result, the PRL content in their blood plasma after the qigong master’s transmission of qi has evidently increased. The PRL content is increased to twice that amount before they receive external qi (P＜0.05). (See Table 1)
2. The influence of restricted stress reaction on the secretion of PRL and the influence of qigong external qi on the secretion of PRL in the restricted stress reaction state.
|Before Qi Transmission||5||4.6±0.5||＜0.05|
|After Qi Transmission||5||10.1±1.9|
Table1 Influence of Qigong External Qi on the Secretion of PRL
in the Resting Rats
The PRL content in the plasma of the first small group of rats of the second big
group becomes obviously increased after the stress reaction. It has increased five
times the PRL content before the stress reaction (P＜0.02). The second small group of rats receive qi after the stress reaction from the qigong master who transmits qi to them with the mental instruction of reducing their PRL. As a result, no evident increase occurs in the PRL content in the blood plasma of this second small group of rats. In comparison with the PRL content in the plasma of this group before the stress reaction, the change in the PRL content shows no statistical significance. Yet in comparison with the PRL content of the first small group after the stress reaction, P value is less than 0.01 (P＜0.01). (See Table 2)
|Group||Time||n||PRL (ng/ml, x±SE)||P|
|1||Before the Stress Reaction||6||10.9±3.2||＜0.02|
|After the Stress Reaction||6||43.8±10.1|
|2||Before the Stress Reaction||6||13.0±2.2||NS|
|After the Stress Reaction plus Qi Transmission||6||10.4±0.8|
Table 2 Influence of Restricted Stress Reaction on the Secretion of PRL and
Influence of Qigong External Qi on the Secretion of PRL in the Re-
stricted Stress Reaction
In recent years, research into the nature of qigong external qi and into the biological
effect it produces has been made in many a subject from many a perspective. Yet it is still difficult to judge whether the external qi transmitted from the human body in the qigong state belongs in electric effect, magnetic effect or infrasonic sound effect or whatsoever. Nevertheless, from clinical observation and experimental observation, the biological effects of qigong external qi objectively exist. Therefore this experiment also tries to make a preliminary exploration into this objective reality.
From the result of our experiment, it can be clearly seen that the qigong master transmits qi to the two big groups of rats respectively with the mental instruction to increase the PRL in their still state and decrease the PRL after they have experienced stress reaction. After the transmission of qi, the PRL content in the plasma of the rats in the calm state becomes evidently increased (P＜0.05), while the PRL content level after the stress reaction slightly decreases instead of increase. This shows that qigong external qi can enhance the secretion of PRL in rats in the calm state and inhibit the release of PRL in rats when they are in a restricted stress reaction state. As to through what functional mechanism qigong external qi produces the above-mentioned effect, it needs further exploration.
In view of the result mentioned above, we deem that a certain amount qigong external qi can influence the secretion of PRL in rats in a calm state and the release of PRL in rats in a stress reaction state. Yet it is worth mentioning that in our series of experimental work, we have seen that different qigong masters, though transmitting the same kind of qigong external qi, produce different biological effects (Data of these experiments not provided here). Therefore, we think that, with further development in the research into qigong external qi, if we can have relatively explicit definition of the attribute of the external qi and there can be relatively better objective index in the method of measurement, research into the medical and biological effects of qigong external qi will become further improved.
- Yang Xiaojie, et al., Experimental Observation of the Influence of Special and Super Human Body Radiation (the Qigong State) upon the Division of Cell Hela, thesis presented in The Third Session of National Academic Conference on the Science of Human Body held by Chinese Human Body Science Society, 1992, p86.
- Tao Huiran, et al., Preliminary Research into the Therapeutic Effect of Qigong External Qi on the Rats’ Alloxan Characteristic Diabetes, thesis presented in The Third Session of National Academic Conference on the Science of the Human Body held by Chinese Human Body Science Society, 1992, p78.
- Xu Rongkun, et al., Preliminary Exploration into the Effect and the Functioning Mechanism of Adrenaline on the Secretion of Adrenocorticotropic Hormone, Journal of China’s Academy of Medical Science, 1989: 9(3):193
- Xu Rongkun, et al., Influence of S-Opiate Receptor Agonist DADLE on the Release of B-Endorphin and Prolactin. Basic and Clinical, 1990: 10(4):31