Effects of External Qi on Transplanted Sarcoma S180 in Mice

Basic Medical Science Research Institute of China’s Academy of Medical Science

Li Mei (1) Zhang Shifu Yun Junti (2) Liu Tong

Hua Xia Zhineng Qigong Training Center Su Dongyue



Transplanted sarcoma S180 in the mice are taken as the samples of tumor in this experiment. The growth of tumor is evidently inhibited under the effect of external qi in the experiment group.

Key words

Zhineng Qigong external qi, transplanted sarcoma S180 in mice

This research aims at exploring Zhineng Qigong external qi’s tumor inhibition effect on the transplanted sarcoma S180 in mice. The experimental result shows that Zhineng Qigong external qi has an evident inhibiting effect on tumor. The rate of tumor inhibition has reached 84.4 percent.


Material and Method

Animals in experiment: Kunming mice multiplied in the Operation Research Institute of China’s Academy of Medical Science are chosen for the experiment. The mice, both male and female, are 10g to 20g in weight and are randomly divided into the external qi group, the short-wave infrared ray qigong instrument irradiation group and the control group. Each group has ten mice.

S180 tumor strains are provided by Pharmaceutical Research Institute of China’s Academy of Medical Science. Dilute ascites S180 in the proportion of 1:4 under an aseptic condition and inoculate each animal in the armpit with a subcutaneous injection of 0.1ml (1×106 tumor cells). 14 hours after the inoculation, transmit external qi to the experiment group. The qigong master sits in front of the cage of mice and sends external qi to them at a distance of 50cm from them. The transmission of qi is carried out one time a day and each transmission lasts for about 17min. The qigong master transmits qi for altogether 12 times. In the short-wave infrared ray group, irradiation goes on at the same time. The dosage of irradiation is 2mm/cm2. It is carried out once a day and each irradiation lasts for an hour. The control group is not treated by any special process. The animals of the experiment group and the control group are fed separately.

In the second week, the tumors are taken out of the mice and are weighted. The tumor inhibition rate is calculated according to formula. The tumor tissues are fixed respectively with solution Bouin and glutaric dialdehyde. They are examined with light microscope and electronic microscope.



External qi has an obvious inhibition effect on S180. The tumor inhibition rate is

84.4 percent (See the table below).

Tumor Inhibition Rate=Weight of tumor in the control group-Weight of tumor in the experiment group ×100%

Weight of tumor in the control group

Morphological Observation

Under the light microscope, it can be seen that the tumor cells in the control group exhibit typical malignant features. These tumor cells vary in their shape. The nucleocytoplasmic ratio is big. They have many different kinds of cell nucleus division phase whereas the cytoplasm in the external qi groups is vacuolate. The cell nuclei are not intact or are broken. The mesenchyme is hyperemic and is infiltrated by many lymphocytes.

Group Number of Animal

Beginning End

Weight of

Tumor (g)


Rat of Tumor Inhibition(%) P Value

Short-wave Infrared

External Qi

10 10

10 10

10 10








Table 1 Tumor Inhibition Effect of Qigong External Qi on S180

With the electron microscope, it can be observed that nucleocytoplasmic ratio of the tumor cells in the control group is big. Volutin agglutinates in the cell nuclei. While the tumor cells in the external qi group show evident degeneration. Mitochondria become vacuolated. The cytoplasm still has the remnants of the fragments of cytoplasm.

In the process of experiment, it is discovered that the formation of tumor in the external qi group is two days later than that in the control group. When it is finally the time for taking the tumor out of the mice, no tumor is found in five mice in the qigong group. In the control group, tumors are found in all the mice. In the short-wave infrared ray qigong instrument positive control group, four mice have at last no tumor formed in them.



The result of this research shows that external qi evidently inhibits the transplantedtumor in the experimental animals. This is similar to the result having been reported by quite a few researchers.

The mechanism of this effect still needs further study. To analyze according to the general condition of the animals, for example, the animals are energetic, lustrous in their hair, full of life in their eyes, have good appetite, etc., it is perhaps that, just like human beings, these animals’ adjusting ability in their body is promoted to inhibit the growth of tumor after the qigong masters transmit qi to them.

In sum, qigong science is both an agedly old and a brand-new science. Research into it to facilitate the development of the science of the human body has definitely far-reaching significance. (1992)

Li Mei: Assistant researcher
Yun Junti: Researcher