Zhineng Qigong’s Effect on the Formation of Clone of Cell Hela in Human Cervix Cancer

Basic Medical Science Research Institute of China’s Academy of Medical Science

Gao Cuihua Zhang Jingbo(1) Zu Shuyu

Qiang Liping Liu Jiahong Wang Ximing

DongdanParkInstructionCenter of Beijing Zhineng Qigong Branch

Guo Honglin Xue Shuqin Wang Shuping

Abstract

This experiment takes Cell Hela in human cervix cancer as the experimental subject in order to observe Zhineng Qigong’s inhibiting and killing effect on the tumor cells cultured outside the human body. The result of the experiment shows that: a) Zhineng Qigong can partially kill cells Hela and inhibit their growth before they become adhered to wall and soon after they adhere to wall, thus reducing the number of clones that can finally come into form; b) As for the cells that have already formed into small clones, Zhineng Qigong can evidently inhibit their proliferation and kill part of them, thus reducing the number of clones that can finally have over 50 cells in them.

Key words

Zhineng Qigong, cell Hella, formation of clone

 

Preface

It has been reported that Zhineng Qigong has a therapeutic effect on tumor patients and an inhibitiing effect on experimental tumor. However, due to the difference in materials and in the qigong masters, the results of experiments are not all the same, which consequently leads to different opinions about the truthfulness of qigong. In this experiment, cells Hela in cervix cancer cultured outside the human body are taken as the subject in order to observe the inhibiting and killing effect of Zhineng Qigong on the tumor cells cultured outside the human body. The preliminary result is now reported as follows:

 

Material and Method

The Hela cell lines are kept in cryopreservation and then are revived in our laboratory. They are made to proliferate in the culture medium of RPMI1640 that contains 10 percent of calf serum. Choose cells Hela that grows in increased logarithmic phase. Make them become unicellulars with the digestion of pancreatin and then inoculate them into the aseptic culture vessels (60mm), with 200 cells in each culture vessel. Culture them in the condition of 370C, 5%CO2. The culture vessels are randomly divided into three groups: the control group, Li Jiahong (qigong master) qi transmission group, Wang Ximing (qigong master) qi transmission group. The whole experiment is carried out in two parts. The first part: On the day when cells Hela are inoculated, the qigong masters transmit qi to them before they adhere to wall. The qi transmission goes on for three days, with one transmission of qi per day. In each qi transmission, the qigong masters send qi to the culture vessels for 10 to 30 min with their mind and observe Zhineng Qigong’s inhibiting and killing effect on cells Hela before they become adhered to wall and soon after they become adhered to wall. The second part: On the fourth and the fifth day after the inoculation of cells Hela when they have grown into the small clones with about 20 cells, qigong masters begin to transmit qi to them. The qi transmission goes on for three days with one transmission of qi each day. In each qi transmission, two to three qigong masters organize qi field and transmit qi to the culture vessels for 10 to 30 minutes with their mind and observe the inhibiting and killing effect of Zhineng Qigong on the cells that have already formed into clones and are vigorously growing. During the whole experiment, we observe the clones and the form of cells in them under the microscope each day. After 11 days of culture, the clones in the culture vessels are colored with Giemsa and the number of clones that have over 50 cells is counted.

 

Result and Discussion

Zhineng Qigong’s inhibiting and killing effect on cells Hela that have not become adhered to wall: The result of the experiment is shown in Table 1. This part of the experiment is carried out in three experimental batches. In the first experimental batch, the cells in the three culture vessels of Liu Jiahong qi transmission group fail to grow into cell groups; the cells in Wang Ximing qi transmission group reduce in their number of cell groups. In the second and the third batches of the experiment, the clones in the culture vessels of the two qigong transmission groups both decrease in number. This shows that Zhineng Qigong can inhibit and kill in part the cells Hela that have not adhered to wall and can prevent theses cells from forming into clones so that the number of clones in the qigong transmission groups is reduced.

Group Vessels of Cells Number of Clone with over 50 Cells

in Each Vessel

P Value
Control Group 15 111.8±15.32
Liu Jiahong Qi Transmission Group 15 57.5±32.27 <0.01
Wang Ximing Qi Transmission Group 15 77.0±12.03 <0.01

Table 1 The Inhibiting and Killing Effect of Zhineng Qigong on Cells Hela that Have Not Adhered to Wall

Zhineng Qigong’s inhibiting and killing effect on cells Hela that have already formed into small clones.

1. Zhineng qigong’s effect on the size of clone and the shape of cell in the clone: After three qi transmissions, there are both in the two qigong groups some clones in the two qigong transmission groups are in bad shape. Fig. 1 shows the clone and the cell shape in the control group and the qigong groups on the 9th day of culture (when the qigong transmission groups have given three times of qi transmission). The clone of cell Hela in the control group has grown into a clone with over 50 cells. The cell boundary is clear and more phenomena of cell divisions can be found, which shows that the cells are exuberantly multiplying, while in the qigong group the number of cells in the clone is still around 20. The cells have not proliferated. Besides, the cells shrink and contract and their boundary is not clear. Their cytoplasm is turbid. Some of the cells are already broken and dead. Some only show the trail of dead cells. This shows that Zhineng Qigong has a partially inhibiting and killing effect on cells Hela that have grown into small clones.

2. The influence of Zhineng Qigong on the number of clones that have finally grown into big clones (Result is shown in Table 2): The experiment is also carried out in three batches, and the results are relatively identical. After the culture of 11 days, there are in each vessel averagely 108 clones with over 50 cells in the control group, while there are about only 80 clones with over 50 cells in each vessel in the qigong group. This shows that there are about 30 small clones in each vessel of the qigong group that have been inhibited or killed in their growing process and fail to grow into the clones with over 50 cells. Zhineng Qigong can inhibit the formation of the clones of cell Hela.

Group Vessels of Cells The Number of Cell Massthat Have over 50 cells in Each Vessel P Value
Control Group 16 108.1±12.60
Liu Jiahong Qi Transmission Group 16 80.2±15.83 <0.01
Wang Ximing Qi Transmission Group 16 81.8±14.47 <0.01

Table 2 Zhineng Qigong’s Inhibiting and Killing Effect on Cells Hela

that Have already Formed into Small Clones

 

Fig. 1 Zhineng Qigong’s Effect on Cell Hela Clones and on the Shape of the Cell in the Clones (on the ninth day after the inoculation of the cell, and the qigong group has been given qi transmission for three times. ×100)

a. Clones in the Control Group b. Clones in the Qigong Group

One tumor cell can generally grow into a clone with over 50 cells within ten to 14 days after it becomes adhered to the wall. If it is killed before or shortly after it be- comes adhered to the wall, the tumor cell will not be able to grow into a clone. Or if, instead of being killed, the tumor cells are but inhibited in their growth, the number of clones they form will be small. The result of the first part of our experiment shows that Zhineng Qigong can partially kill or inhibit cells Hela before or shortly after they become adhered to the wall and can reduce the number of clones that finally come into form. Then we take a step further and ask the qigong masters to transmit qi to the cells Hela that have already grown into small clones with 20 cells in each and are at a time of vigorous growth. The result shows that Zhineng Qigong also has an inhibiting and killing effect on them and reduces the number of clones that finally grow into clones with over 50 cells. The result of the two parts of experiment show that Zhineng Qigong has an effect of inhibiting and killing cells Hela cultured outside the human body. We have also made a comparison of qi transmission that is conducted by several qigong masters working together and by a single qigong master working alone. The killing effect on cells Hela by qigong masters uniting together is stronger than by one single qigong master. As for the functioning mechanism of Zhineng Qigong killing tumor cell, we have preliminarily observed that it is probably apoptosis of cells Hela. But it still needs proof with further experiment.