Basic Medical Science Research Institute of China’s Academy of Medical Science
Li Mei（1） Zhang Shifu（2） Liu Tong
Hua Xia Zhinene Qigong Training Center Su Dongyue
This experiment aims at observing Zhineng Qigong’s effect on the multiplication of mice’s erythroleukemia cell line (MEL) that is cultured outside their body. The result of the experiment proves that the cell multiplication in the experiment group is evidently inhibited.
Zhineng Qigong external qi, growth curve, cells of erythroleukemia (MEL) in mice
Cell culture technique is adopted in this experiment to observe the tumor inhibition effect of Zhineng Qigong upon mice’s erythroleukemia(MEL) cultured outside the mice’s body. The major index of observation is the effect of external qi on the multiplication of cell line of MEL tumor.
Material and Method
Tumor Strain: Mice’s erythroleukemia (MEL) cultured outside the mice’s body. They are kept in cryopreservation and revived in our laboratory.
Condition of culture: The cell culture medium is DMEM that contains 10% calf blood serum. Each milliliter of the culture medium contains 100 international unit penicillin and 100 microgram streptomycin. The tumor cells are cultured in the incubation box with 5%CO2. The culture temperature is kept at 370C.
Make cell suspension out of the MEL cells that are growing well. Color them with trypan blue before counting the number of cells and divide them into different bottles. The experiment is repeated for twice. The first time: Culture the cells in big glass bottles; the number of cells is 10×104 in each milliliter of cell medium. The second time: Use small glass bottles of 25ml; the number of cells is 3×104 per milliliter of culture medium. We arrange experiment group and control group in both these two times of experiments, with 10 bottles of cells in each group. Qigong masters stands in front of the bottles and transmits external qi to the experiment group 24 hours after the cell suspension is divided into the ten bottles. He transmits qi once a day. Each transmission lasts averagely about 18 min. The control group receives no special process of treatment. The number of living cells and dead cells in the two groups are counted everyday. Each day, cells in two bottles are computed and we obtain the average of MEL cells in each milliliter of culture medium everyday to observe the influence of external qi on the proliferation of MEL cells.
The results of the two experiments are similar. Both show the evident inhibiting and killing effect of external qi on MEL cells cultured outside thee mice’s body. Now the result of the second experiment is drawn into a growth curve. The numbers of the multiplication of MEL cells each day in the two experiments are compared in the following table.
|Times of Experiment||Days of Culture||Number of Cells/ml||Time that the Qigong Master Spent in Sending External Qi (min)|
|Control Group||Experiment Group|
|The First Time||1
|The Second Time||1
Table 1 Effect of Zhineng Qigong on the Growth of MEl Cells
The growth curve indicates that the multiplication of cells MEL in the second experiment is evidently affected by Zhineng Qigong and shows a tendency of going down directly. In the first experiment, though fluctuations occur, the number of cells in Zhineng Qigong group on the 6th day is 4×104 per milliliter culture medium, while that in the control group is 363×104 per milliliter culture medium. Everyday, afterMEL are colored with trypan blue，it can be seen that there are more dead cells in the qigong group when the number of cells are counted. As for the mechanism of Zhineng Qigong inhibiting and killing cells MEL, it needs further study.
In the process of the experiment, we perceive that qigong master’s mental instruction plays an important and magic role in influencing the growth of tumor cells. Mental instruction is an activity that is going on in the human brain; it is material. Yet how should we check and measure its biological effect? What kind of material the mental activity in the qigong state is? What is the standard for its dosage? How should we measure the dosage? All these questions deserve further exploration.
The index that is measured in this experiment is relatively simple. It is just a preliminary observation and is for reference only. (1992)
Li Mei: Assistant researcher
Zhang Shifu: Assistant researcher